Journal of Physiological Sciences 2015-11-01

Moderate dependence of reactive oxygen species production on membrane potential in avian muscle mitochondria oxidizing glycerol 3-phosphate.

Motoi Kikusato, Masaaki Toyomizu

Index: J. Physiol. Sci. 65 , 555-9, (2015)

Full Text: HTML

Abstract

Mitochondria are a major source of reactive oxygen species production in cells, and the production level is sensitive to the magnitude of the membrane potential (ΔΨ). The present study investigated the level of superoxide production in mitochondria oxidizing glycerol 3-phosphate (GP) and its dependence on ΔΨ in isolated avian muscle mitochondria. The levels of superoxide produced in mitochondria oxidizing GP were lower than those obtained with succinate and were similar to those obtained with NADH-linked substrates (glutamate/malate/pyruvate). The dependence of superoxide production on ΔΨ in mitochondria oxidizing GP was lower than that of mitochondria oxidizing succinate, and a weak dependence of GP-supported superoxide production on ΔΨ was observed in the presence of NADH-linked substrates or succinate. These results suggest that the levels of superoxide generated in response to GP are quantitatively low, but they are unsusceptible to changes in ΔΨ in avian muscle mitochondria.

Related Compounds

  • Hydrogen peroxide
  • EGTA
  • Magnesium choride
  • Ampliflu Red

Related Articles:

Neuropeptide Y in the noradrenergic neurones induces obesity and inhibits sympathetic tone in mice.

2015-04-01

[Acta Physiol. (Oxf.) 213(4) , 902-19, (2015)]

G-protein-coupled estrogen receptor 1 is anatomically positioned to modulate synaptic plasticity in the mouse hippocampus.

2015-02-11

[J. Neurosci. 35(6) , 2384-97, (2015)]

The Drosophila MAPK p38c regulates oxidative stress and lipid homeostasis in the intestine.

2014-09-01

[PLoS Genet. 10(9) , e1004659, (2014)]

Driving cartilage formation in high-density human adipose-derived stem cell aggregate and sheet constructs without exogenous growth factor delivery.

2014-12-01

[Tissue Eng. Part A 20(23-24) , 3163-75, (2014)]

Diclofenac toxicity in human intestine ex vivo is not related to the formation of intestinal metabolites.

2015-01-01

[Arch. Toxicol. 89(1) , 107-19, (2015)]

More Articles...