Journal of Biological Chemistry 2003-09-05

Hormonal control of ADP-ribosyl cyclase activity in pancreatic acinar cells from rats.

Lutz Sternfeld, Elmar Krause, Andreas H Guse, Irene Schulz

Index: J. Biol. Chem. 278(36) , 33629-36, (2003)

Full Text: HTML

Abstract

Cyclic ADP-ribose, a metabolite of NAD+ evokes Ca2+ release from intracellular stores in different cells. We have determined the activity of cADPr-producing enzymes (ADP-ribosyl cyclases) in different cellular fractions prepared from isolated pancreatic acinar cells by measuring the conversion of the beta-NAD+ analogs 1,N6-etheno-NAD and nicotinamide guanine dinucleotide to the fluorescent products 1,N6-etheno-cADPr and cyclic GDP-ribose, respectively. Substrate/product analyses were carried out by reverse-phase high pressure liquid chromatography. In all subcellular fractions examined (cytosol, mitochondria, plasma, and intracellular membranes), ADP-ribosyl cyclase activity was detected except in zymogen granular membranes. Western blot analysis and immunoprecipitation experiments revealed the presence of the ADP-ribosyl cyclase CD38 in both plasma membranes and mitochondria but not in the cytosol. Hormonal stimulation of intact acinar cells for 1 min with acetylcholine (ACh), cholecystokinin (CCK), or a membrane-permeant analog of cGMP increased ADP-ribosyl cyclase activity in the cytosol by 1.8-, 1.6-, and 1.9-fold, respectively, as compared with the control but had no effect in any other fraction. Both ACh and CCK also increased accumulation of cGMP in the cells by about 2-fold. Bombesin had no significant effect on either ADP-ribosyl cyclase activity or cGMP accumulation within this short period of stimulation. We conclude that at least two types of ADP-ribosyl cyclases are present in pancreatic acinar cells: membrane-bound CD38 and a cytosolic enzyme different from CD38. Stimulation of pancreatic acinar cells with CCK or ACh results in exclusive activation of the cytosolic ADP-ribosyl cyclase activity, most likely mediated by cGMP.


Related Compounds

  • nicotinamide guani...
  • Nicotinamide 1,N6...

Related Articles:

ADP-ribosyl cyclase and GDP-ribosyl cyclase activities are not always equivalent: impact on the study of the physiological role of cyclic ADP-ribose.

2005-11-15

[Anal. Biochem. 346(2) , 336-8, (2005)]

Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in bovine rod outer segments.

2007-03-01

[Invest. Ophthalmol. Vis. Sci. 48 , 978-984, (2007)]

Purification and partial characterization of a novel phosphodiesterase from the venom of Trimeresurus stejnegeri: inhibition of platelet aggregation.

2011-09-01

[Biochimie 93 , 1601-1619, (2011)]

Catalytic properties of the retinal rod outer segment disk ADP-ribosyl cyclase.

2011-03-01

[Vis. Neurosci. 28 , 121-128, (2011)]

The CD38-independent ADP-ribosyl cyclase from mouse brain synaptosomes: a comparative study of neonate and adult brain.

2006-04-15

[Biochem. J. 395(2) , 417-26, (2006)]

More Articles...