2-Methoxy-4-nitroaniline is a selective inducer of cytochrome P450IA2 (CYP1A2) in rat liver.

M Degawa, M Nakayama, K Yoshinari, Y Hashimoto

Index: Cancer Lett. 96(1) , 95-8, (1995)

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Abstract

Male F344 rats were treated with a chemical (aniline, nitrobenzene, 2-methoxy-p-phenylenediamine, 2-methoxy-4-nitroaniline or 2-methoxy-4-nitroazobenzene) produced by the azo-reduction and/or N-oxidation of 2-methoxy-4-amino-azo-benzene, a selective inducer of cytochrome P450IA2 (CYP1A2), and their effects on the induction of CYP1A enzymes in the liver were examined in terms of the protein and mRNA amounts. 2-Methoxy-4-nitroaniline and 2-methoxy-4-nitroazo-benzene, but not other compounds tested, induced CYP1A enzymes, especially CYP1A2, as assessed by Western blotting and Northern blotting. It is noteworthy that 2-methoxy-4-nitroaniline was more selective than 2-methoxy-4-aminoazobenzene for induction of CYP1A2, and it has the smallest molecular size among the known CYP1A2 inducers.