Journal of Pharmaceutical and Biomedical Analysis 1994-01-01

Mixed ion pair liquid chromatography method for the simultaneous assay of ascorbic acid, caffeine, chlorpheniramine maleate, dextromethorphan HBr monohydrate and paracetamol in Frenadol sachets.

B R Thomas, X G Fang, P Shen, S Ghodbane

Index: J. Pharm. Biomed. Anal. 12(1) , 85-90, (1994)

Full Text: HTML

Abstract

The five active drug substances and two of the excipients present in Frenadol, a cold medication, were separated. The active drug components dextromethorphan HBr monohydrate, ascorbic acid, caffeine, paracetamol and chlorpheniramine maleate were quantitatively assayed by a mixed ion pair LC method. The excipients separated were citric acid and maleic acid. The HPLC assay included dual-wavelength detection to simultaneously quantify the large concentration of paracetamol and the much lower concentration of chlorpheniramine and dextromethorphan. Both tetrabutylammonium hydrogen sulphate (TBA) and pentane sulphonic acid (PSA) were necessary for resolution of the seven compounds. The TBA was necessary to lessen peak tailing for dextromethorphan and chlorpheniramine, to retain ascorbic acid and to shorten assay time. The pentane sulphonic acid enhanced peak shape for dextromethorphan and chlorpheniramine. The assay of the active drug substances was validated for use in quality control applications. Validation studies demonstrated that the procedure was accurate, linear, precise, reproducible and rugged. The method conformed to both USP and EC validation guidelines.


Related Compounds

  • Sodium 1-pentanesu...

Related Articles:

Development and validation of a stability-indicating LC-UV method for the determination of pantethine and its degradation product based on a forced degradation study.

2014-08-01

[J. Pharm. Biomed. Anal. 97 , 141-50, (2014)]

Rapid and selective micellar electrokinetic chromatography for simultaneous determination of amikacin, kanamycin A, and tobramycin with UV detection and application in drug formulations.

2005-02-01

[Electrophoresis 26(4-5) , 947-53, (2005)]

Effects of pulsed ultrasound on the adsorption of n-alkyl anionic surfactants at the gas/solution interface of cavitation bubbles.

2007-02-15

[J. Phys. Chem. B 111(6) , 1361-7, (2007)]

A comparative sonochemical reaction that is independent of the intensity of ultrasound and the geometry of the exposure apparatus.

2008-09-01

[Ultrason. Sonochem. 15(6) , 1043-8, (2008)]

The solubility of ethane in aqueous solutions of sodium 1-pentanesulfonate, sodium 1-hexanesulfonate, sodium 1-heptanesulfonate, and sodium 1-octanesulfonate at 25 degrees C.

2007-05-15

[J. Colloid. Interface Sci. 309(2) , 505-10, (2007)]

More Articles...