Biotechnology Letters 2012-10-01

Metabolic engineering of Bacillus subtilis for enhanced production of acetoin.

Meng Wang, Jing Fu, Xueyu Zhang, Tao Chen

Index: Biotechnol. Lett. 34(10) , 1877-85, (2012)

Full Text: HTML

Abstract

Acetoin is widely used in food and other industries. A bdhA and acoA double-knockout strain of Bacillus subtilis produced acetoin at 0.72 mol/mol, a 16.4 % increased compared to the wild type. Subsequent overexpression of the alsSD operon enhanced the acetolactate synthase activity by 52 and 66 % in growth and stationary phases, respectively. However, deletion of pta gene caused little increase of acetoin production. For acetoin production by the final engineered strain, BSUW06, acetoin productivity was improved from 0.087 g/l h, using M9 medium plus 30 g glucose/l under micro-aerobic conditions, to 0.273 g/h l using LB medium plus 50 g glucose/l under aerobic conditions. In fermentor culture, BSUW06 produced acetoin up to 20 g/l.

Related Compounds

  • meso-2,3-butanedio...
  • Acetoin
  • (2S,3S)-(+)-2,3-B...
  • (2R,3R)-(-)-2,3-B...
  • (2R,3R)-(-)-2,3-B...

Related Articles:

The genome of Pelobacter carbinolicus reveals surprising metabolic capabilities and physiological features.

2012-01-01

[BMC Genomics 13 , 690, (2012)]

Identification of factors regulating Escherichia coli 2,3-butanediol production by continuous culture and metabolic flux analysis.

2012-05-01

[J. Microbiol. Biotechnol. 22(5) , 659-67, (2012)]

Medium optimization and proteome analysis of (R,R)-2,3-butanediol production by Paenibacillus polymyxa ATCC 12321.

2013-01-01

[Appl. Microbiol. Biotechnol. 97(2) , 585-97, (2013)]

Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues.

2012-10-01

[Cryobiology 65(2) , 117-25, (2012)]

Biocatalytic production of alpha-hydroxy ketones and vicinal diols by yeast and human aldo-keto reductases.

2013-02-25

[Chem. Biol. Interact. 202(1-3) , 195-203, (2013)]

More Articles...