Bioorganic & Medicinal Chemistry Letters 1998-11-03

Evaluation of resins for on-bead screening: a study of papain and chymotrypsin specificity using PEGA-bound combinatorial peptide libraries.

S Leon, R Quarrell, G Lowe

Index: Bioorg. Med. Chem. Lett. 8(21) , 2997-3002, (1998)

Full Text: HTML

Abstract

TentaGel, ArgoGel and PEGA resins were evaluated for on-bead biological screening, using a fluorescently-labelled peptide attached to each and assayed for papain activity. Peptide attached to PEGA was cleaved in near quantitative yield at the expected sites, whilst an identical sequence on TentaGel and ArgoGel beads was hydrolysed in very low yields and nonspecifically on ArgoGel. The compatibility of PEGA with enzymes was further demonstrated by the determination of subsite specificities of papain and chymotrypsin using PEGA-bound peptide libraries, which proved to be similar to those observed in free solution.

Related Compounds

  • Polyethylene glyco...

Related Articles:

Synthesis of semitelechelic maleimide poly(PEGA) for protein conjugation by RAFT polymerization.

2009-07-13

[Biomacromolecules 10(7) , 1777-81, (2009)]

Construction of highly glycosylated mucin-type glycopeptides based on microwave-assisted solid-phase syntheses and enzymatic modifications.

2006-04-14

[J. Org. Chem. 71(8) , 3051-63, (2006)]

Solid-phase library synthesis, screening, and selection of tight-binding reduced peptide bond inhibitors of a recombinant Leishmania mexicana cysteine protease B.

2002-05-09

[J. Med. Chem. 45(10) , 1971-82, (2002)]

Improved biotransformations on charged PEGA supports.

2003-06-07

[Chem. Commun. (Camb.) (11) , 1296-7, (2003)]

Characterization of modified alginate-poly-L-lysine microcapsules.

1997-09-01

[Artif. Organs 21(9) , 1002-6, (1997)]

More Articles...