Highly selective ratiometric fluorescent probe for Au3+ and its application to bioimaging.

Ji Young Choi, Gun-Hee Kim, Zhiqian Guo, Hye Yeon Lee, K M K Swamy, Jaeyoung Pai, Seunghoon Shin, Injae Shin, Juyoung Yoon

Index: Biosens. Bioelectron. 49 , 438-41, (2013)

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Abstract

The 4-propargylamino-1,8-naphthalimide based fluorescent probe 1 has been explored as a sensor for selective detection of Au(3+). 4-Amino-1,8-naphthalimides, that possess typical intramolecular charge transfer (ICT) electronic characteristics, have been widely used as versatile platforms for fluorescent probes. The newly designed probe 1 contains a propargylamine moiety at C-4 of the naphthalimide chromophore that reacts with Au(3+) to generate a product that has distinctly different electronic properties from 1. Specifically, the probe undergoes a remarkable change in its absorption spectrum upon addition of Au(3+) that is associated with a distinct color change from yellow to light pink. In addition, a blue shift of ca. 56 nm also takes place in the emission spectra of the probe. Consequently, 1 serves as a reaction-based sensor or so called chemodosimeter for Au(3+). Importantly, surfactants enhance the rate of reaction of 1 with Au(3+), thus, enhancing its use as a real time sensor. Finally, the results of studies probing its application to bioimaging of Au(3+) in live cells show that the probe 1 has a unique ability to sense Au(3+) in cells and, in particular, in lipid droplets within cells.Copyright © 2013 The Authors. Published by Elsevier B.V. All rights reserved.