Journal of Chromatography B 2011-09-15

Quantification of the antifungal lipopeptide iturin A by high performance liquid chromatography coupled with aqueous two-phase extraction.

Jun Yuan, Waseem Raza, Qiwei Huang, Qirong Shen

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 879(26) , 2746-50, (2011)

Full Text: HTML

Abstract

Iturin A, a powerful antifungal surfactant, is a kind of bacterial lipopeptide produced by Bacillus strains. This study addresses the use of an aqueous two-phase system (ATPS) using ethanol/ammonium sulfate to extract iturin A from Bacillus amyloliquefaciens NJN-6 fermentation broth and the quantification of iturin A by HPLC. Baseline separation of iturin A homologues was performed using an RP-C(18) column with a mixture of water and acetonitrile. The results showed that the correlation coefficient between integral area and concentration was 0.9961 within the range of 20-140 mg/l. The RSD of the retention time and the peak area were 1.29% and 1.45%, respectively. The effects of some operating parameters in ATPS, e.g., pH, temperature and centrifugation time, were also studied. This method can be successfully used for the rapid quantification of iturin A.Copyright © 2011 Elsevier B.V. All rights reserved.

Related Compounds

  • Iturin A

Related Articles:

Study of the antifungal activity of Acinetobacter baumannii LCH001 in vitro and identification of its antifungal components.

2007-08-01

[Appl. Microbiol. Biotechnol. 76(2) , 459-66, (2007)]

Production of lipopeptides in Bacillus sp. CS93 isolated from Pozol.

2010-03-01

[FEMS Microbiol. Lett. 304(1) , 69-73, (2010)]

Microbial analysis of a composted product of marine animal resources and isolation of bacteria antagonistic to a plant pathogen from the compost.

2008-06-01

[J. Gen. Appl. Microbiol. 54(3) , 149-58, (2008)]

Production of biosurfactant lipopeptides Iturin A, fengycin and surfactin A from Bacillus subtilis CMB32 for control of Colletotrichum gloeosporioides.

2010-01-01

[J. Microbiol. Biotechnol. 20 , 138-145, (2010)]

Cell surface hydrophobicity of Bacillus spp. as a function of nutrient supply and lipopeptides biosynthesis and its role in adhesion.

2008-01-01

[Pol. J. Microbiol. 57(4) , 313-9, (2008)]

More Articles...