Journal of Lipid Research 1995-08-01

Fluorometric detection of glycosphingolipids on thin-layer chromatographic plates.

K Watanabe, M Mizuta

Index: J. Lipid Res. 36(8) , 1848-55, (1995)

Full Text: HTML

Abstract

A microdetection system for glycosphingolipid analysis has been developed using 5-hydroxy-1-tetralone as the fluorescent labeling reagent. The reagents in H2SO4 permit the fluorometric detection of acidic and neutral glycosphingolipids both in test tube and on thin-layer chromatographic plates. Glycosphingolipids can be detected at concentrations as low as 5 pmol on the thin-layer chromatographic plate. The method is a rapid and simple, and feasible for determination of glycosphingolipid from small amounts of biological samples.

Related Compounds

  • 5-Hydroxy-1-tetral...

Related Articles:

Simple and selective assay of 4-hydroxymephenytoin in human urine using solid-phase extraction and high-performance liquid chromatography with electrochemical detection and its preliminary application to phenotyping test.

1996-02-09

[J. Chromatogr. B, Biomed. Appl. 676(1) , 87-94, (1996)]

Studies on Quinones. Part 22.'Synthesis of 1-Benzazepine-6, 9-Quinone Derivatives. Valderrama JA, et al.

[Synth. Commun. 22(4) , 629-40, (1992)]

A new chiral oxathiane: synthesis, resolution and absolute configuration determination by vibrational circular dichroism. Solladié-Cavallo A, et al.

[Tetrahedron Asymmetry 12(18) , 2605-11, (2001)]

More Articles...