Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel.

Swagata Das, Biswajit Mishra, Kamaldeep Gill, Md Saquib Ashraf, Abhay Kumar Singh, Mou Sinha, Sujata Sharma, Immaculata Xess, Krishna Dalal, Tej Pal Singh, Sharmistha Dey

Index: Int. J. Biol. Macromol. 48(1) , 38-43, (2011)

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Abstract

The Aloe protein of 14 kDa from the Aloe vera leaf gel was isolated by an ion exchange chromatography using DEAE-cellulose and CM-cellulose column. The purified Aloe protein exhibited a potent anti-fungal activity against Candida paraprilosis, Candida krusei and Candida albicans. In addition, the purified Aloe protein also showed an anti-inflammatory property against pure lipoxygenase and cyclooxygenase-2 with 84% and 73% inhibition, respectively, and was verified by binding with these proteins by real time method by the phenomenon of surface plasmon resonance. This Aloe protein is a novel protein possessing antifungal and anti-inflammatory properties and thus sets a platform to be used as a medicinal plant product.Copyright © 2010 Elsevier B.V. All rights reserved.