Analysis of Salvinorin A in urine using microextraction in packed syringe and GC-MS/MS.

Ivo Moreno, Beatriz da Fonseca, David Oppolzer, Ana Martinho, Mário Barroso, Angelines Cruz, João António Queiroz, Eugenia Gallardo

Index: Bioanalysis 5(6) , 661-8, (2013)

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Abstract

The aim of this work was to develop and validate a method for the determination of Salvinorin A in human urine using microextraction by packed sorbent (MEPS) and GC-MS/MS.The technique uses a sample volume as low as 0.2 ml, and the analyte was extracted using a C18 sorbent. The method showed to be linear between 20 and 1000 ng/ml and presented a LOD of 5 ng/ml. Intra- and inter-day precision and accuracy were acceptable. Absolute recoveries ranged from 71 to 80%.GC-MS/MS with MEPS demonstrated to be a fast and simple procedure for the quantification of Salvinorin A in urine. This is the first time that GC-MS/MS with MEPS was used for the determination of this compound in biological fluids. Furthermore, the device could be reused for up to 80 extractions, which accounted for a lower cost of analysis.