FEMS Microbiology Letters 1992-09-15

Sequence and functional analysis of the cloned Neisseria meningitidis CMP-NeuNAc synthetase.

U Edwards, M Frosch

Index: FEMS Microbiol. Lett. 75 , 161-166, (1992)

Full Text: HTML

Abstract

The CMP-N-acetylneuraminic acid (CMP-NeuNAc) synthetase gene of Neisseria meningitidis group B is located on a 2.3-kb EcoRI fragment within the cps gene cluster. Nucleotide sequence determination of the gene encoding the CMP-NeuNAc synthetase revealed a 515-bp open reading frame that can encode a 18.9-kDA protein. A computer data base scan revealed a 59.4% identity to the CMP-NeuNAc synthetase gene of E. coli K1. Enzymatic activity was confirmed in vitro and in vivo. Transformation of the CMP-NeuNAc defective E. coli K1 strain EV5 with the meningococcal CMP-NeuNAc synthetase could complement the defect in E. coli.

Related Compounds

  • CYTIDINE 5'-T...

Related Articles:

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination.

2014-12-01

[Cell. Mol. Life Sci. 71(24) , 4895-910, (2014)]

CTP:phosphocholine cytidylyltransferase α (CCTα) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis.

2011-06-01

[Biochim. Biophys. Acta 1811 , 377-385, (2011)]

The Kap60-Kap95 karyopherin complex directly regulates phosphatidylcholine synthesis.

2009-03-13

[J. Biol. Chem. 284 , 7376-7384, (2009)]

Purification of CMP-N-acetylneuraminic acid synthetase from bovine anterior pituitary glands.

1999-05-01

[Glycobiology 9 , 481-487, (1999)]

More Articles...