Biochemical Journal 1986-05-01

Thyrotropin cross-links to the thyrotropin receptor through both the alpha and beta subunits.

P R Buckland, C R Rickards, R D Howells, B R Smith

Index: Biochem. J. 235(3) , 879-82, (1986)

Full Text: HTML

Abstract

We have recently shown that the beta subunit of thyrotropin (TSH) can be cross-linked to the TSH receptor [Buckland, Strickland, Pierce & Rees Smith (1985) Endocrinology (Baltimore) 116, 2122-2124; Buckland, Strickland & Rees Smith (1985) Biochem. Soc. Trans. 13, 942-943]. We failed, however, to cross-link the alpha subunit to the receptor, leaving the role of this subunit in the TSH-TSH-receptor interaction uncertain. We now report the successful cross-linking of the TSH alpha subunit to the receptor by the use of two different cross-linking reagents. Our studies suggest therefore that both subunits of TSH form part of the hormone's receptor-binding site.

Related Compounds

  • ANB-NOS

Related Articles:

Photoactivated heterobifunctional cross-linking reagents which demonstrate the aggregation state of phospholipase A2.

1977-12-13

[Biochemistry 16 , 5650-5654, (1977)]

Covalent binding of biological samples to solid supports for scanning probe microscopy in buffer solution.

1993-12-01

[Biophys. J. 65(6) , 2437-46, (1993)]

[Structural characterization of the somatostatin receptors on rat cerebrocortical membranes].

1990-10-20

[Nihon Naibunpi Gakkai Zasshi 66(10) , 1108-16, (1990)]

Structural characterization of Y1 and Y2 receptors for neuropeptide Y and peptide YY by affinity cross-linking.

1990-05-15

[J. Biol. Chem. 265(14) , 8304-10, (1990)]

Structural characterization of the somatostatin receptor in rat anterior pituitary membranes.

1987-08-01

[Endocrinology 121(2) , 486-92, (1987)]

More Articles...