Development of molecular probes for second-site screening and design of protein tyrosine phosphatase inhibitors.

Jesus Vazquez, Lutz Tautz, Jennifer J Ryan, Kristiina Vuori, Tomas Mustelin, Maurizio Pellecchia

Index: J. Med. Chem. 50 , 2137, (2007)

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Abstract

We report on the design, synthesis, and evaluation of a series of furanyl-salicyl-nitroxide derivatives as effective chemical probes for second-site screening against phosphotyrosine phosphatases (PTPs) using NMR-based techniques. The compounds have been tested against a panel of PTPs to assess their ability to inhibit a broad spectrum of these phosphatases. The utility of the derived compounds is illustrated with the phosphatase YopH, a bacterial toxin from Yersinia pestis. Novel chemical fragments were identified during an NMR-based screen for compounds that are capable of binding on the surface of YopH in regions adjacent the catalytic site in the presence of the spin-labeled compounds. Our data demonstrate the value of the derived chemical probes for NMR-based second-site screening in PTPs.