Journal of Chromatography A 2003-02-14

Determination of herbicides and a metabolite in human urine by liquid chromatography-electrospray ionization mass spectrometry.

Joseane M Pozzebon, Wagner Vilegas, Isabel C S F Jardim

Index: J. Chromatogr. A. 987(1-2) , 375-80, (2003)

Full Text: HTML

Abstract

A method was developed to determine simazine, atrazine and their metabolite, 2-chloro-4,6-diamino-1,3,5-triazine, in urine. The presence of these herbicides in urine may reflect possible exposure to pesticides. Sample preparation involved protein precipitation and solid-phase extraction. The samples were analyzed by high-performance liquid chromatography-mass spectrometry. The detection limits were 0.4 microg/l and the analytes have a linear response in the interval 6-800 microg/l. The precision of the method was reflected in the RSD of < 2.4% for the herbicides studied. Based on the detectable herbicide levels from spiked urine samples collected from unexposed volunteers, this method can be used to determine the low levels necessary for establishing reference values of the selected herbicides and the metabolite.

Related Compounds

  • Deethyldeisopropyl...

Related Articles:

Alteration of catecholamines in pheochromocytoma (PC12) cells in vitro by the metabolites of chlorotriazine herbicide.

2001-01-01

[Toxicol. Sci. 59(1) , 127-37, (2001)]

Pubertal development in female Wistar rats following exposure to propazine and atrazine biotransformation by-products, diamino-S-chlorotriazine and hydroxyatrazine.

2003-11-01

[Toxicol. Sci. 76(1) , 190-200, (2003)]

Oral absorption and oxidative metabolism of atrazine in rats evaluated by physiological modeling approaches.

2007-10-30

[Toxicology 240(1-2) , 1-14, (2007)]

Utilization of chlorinated s-triazines by a new strain of Klebsiella pneumoniae.

1995-01-01

[Appl. Microbiol. Biotechnol. 42(5) , 763-8, (1995)]

Identification of a novel hemoglobin adduct in Sprague Dawley rats exposed to atrazine.

2006-05-01

[Chem. Res. Toxicol. 19(5) , 692-700, (2006)]

More Articles...