Biotechnology Letters 2012-01-01

Cholesterol assimilation and biotransformation by Lactobacillus helveticus.

Jayesh J Ahire, Anuja A Bhat, Jayashri M Thakare, Pallavi B Pawar, Dipali G Zope, Rakeshkumar M Jain, Bhushan L Chaudhari

Index: Biotechnol. Lett. 34(1) , 103-7, (2012)

Full Text: HTML

Abstract

Lactobacillus helveticus, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg(-1) of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.04 g of androst-4-ene-3, 17 dione within 48 h at 37°C with extracellular cholesterol oxidase activity at 12 U mg(-1) and intracellular oxidase at 0.5 U mg(-1).

Related Compounds

  • Cholesterol oxidas...
  • androsta-1,4-diene...

Related Articles:

Phospholipid-sepiolite biomimetic interfaces for the immobilization of enzymes.

2011-11-01

[ACS Appl. Mater. Interfaces 3(11) , 4339-48, (2011)]

Electro-chemiluminescent biosensing.

2008-01-01

[Anal. Bioanal. Chem 390 , 155-68, (2008)]

Effect of cholesterol concentration on organization of viral and vesicle membranes. Probed by accessibility to cholesterol oxidase.

1980-06-25

[J. Biol. Chem. 255(12) , 5802-6, (1980)]

Enzymatic determination of total serum cholesterol.

1974-04-01

[Clin. Chem. 20(4) , 470-5, (1974)]

Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis.

2002-06-01

[Protein Eng. 15(6) , 477-84, (2002)]

More Articles...