Lab On a Chip 2012-07-21

Enriching carbonylated proteins inside a microchip through the use of oxalyldihydrazide as a crosslinker.

Bryant C Hollins, Steven A Soper, June Feng

Index: Lab Chip 12(14) , 2526-32, (2012)

Full Text: HTML

Abstract

We report a proof of principle study for the use of oxalyldihydrazide as a crosslinker for enrichment of carbonylated proteins within a microfluidic chip. Surface modification steps are characterized and analyzed using analytical techniques. We use oxidized cytochrome c as our model protein and demonstrate the chip's ability to capture carbonylated targets. After 100 min of continuous loading, the chip is capable of capturing 7.5 μg of carbonylated protein. All the proteins captured are eluted out of the chip using the elution protocol. Finally, we demonstrate the chip's specificity for oxidized targets by mixing oxidized cytochrome c and TRITC-BSA, with cytochrome c in low abundance. The results show that the chip is efficient at finding its target when unoxidized proteins are present. This is the first report to suggest the use of immobilized oxalyldihydrazide on a microchip as an enrichment methodology for low abundance proteins in a sample.

Related Compounds

  • Ethanedihydrazide

Related Articles:

Efficient Delivery of DOX to Nuclei of Hepatic Carcinoma Cells in the Subcutaneous Tumor Model Using pH-Sensitive Pullulan-DOX Conjugates.

2015-07-29

[ACS Appl. Mater. Interfaces 7 , 15855-65, (2015)]

3-Hydroxypyridinium cross-links in lathyritic tissues.

1981-08-14

[Biochem. Biophys. Res. Commun. 101(3) , 1026-30, (1981)]

Effect of different fuels on structural, thermo and photoluminescent properties of Gd2O3 nanoparticles.

2012-10-01

[Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 96 , 532-40, (2012)]

Antibacterial activity and spectral studies of trivalent chromium, manganese, iron macrocyclic complexes derived from oxalyldihydrazide and glyoxal.

2002-01-01

[J. Enzyme Inhib. Med. Chem. 24(3) , 883-9, (2009)]

Antimicrobial, spectral, magnetic and thermal studies of Cu(II), Ni(II), Co(II), UO(2)(VI) and Fe(III) complexes of the Schiff base derived from oxalylhydrazide.

2008-04-01

[J. Enzyme Inhib. Med. Chem. 23(2) , 285-95, (2008)]

More Articles...