Chlamydia pneumoniae negatively regulates ABCA1 expression via TLR2-Nuclear factor-kappa B and miR-33 pathways in THP-1 macrophage-derived foam cells.

Guo-Jun Zhao, Zhong-Cheng Mo, Shi-Lin Tang, Xin-Ping Ouyang, Ping-Ping He, Yun-Cheng Lv, Feng Yao, Yu-Lin Tan, Wei Xie, Jin-Feng Shi, Yan Wang, Min Zhang, Dan Liu, Deng-Pei Tang, Xi-Long Zheng, Guo-Ping Tian, Chao-Ke Tang

Index: Atherosclerosis 235(2) , 519-25, (2014)

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Abstract

ATP-binding cassette transporter A1 (ABCA1) is critical in exporting cholesterol from macrophages and plays a protective role in the development of atherosclerosis. This study was to determine the effects and potential mechanisms of Chlamydia pneumoniae (C. pneumoniae) on ABCA1 expression and cellular cholesterol efflux in THP-1 macrophage-derived foam cells.C. pneumoniae significantly decreased the expression of ABCA1 and reduced cholesterol efflux. Furthermore, we found that C. pneumoniae suppressed ABCA1 expression via up-regulation of miR-33s. The inhibition of C. pneumoniae-induced NF-κB activation decreased miR-33s expression and enhanced ABCA1 expression. In addition, C. pneumoniae increased Toll-like receptor 2 (TLR2) expressions, inhibition of which by siRNA could also block NF-κB activation and miR-33s expression, and promot the expression of ABCA1.Taken together, these results reveal that C. pneumoniae may negatively regulate ABCA1 expression via TLR2-NF-κB and miR-33 pathways in THP-1 macrophage-derived foam cells, which may provide new insights for understanding the effects of C. pneumoniae on the pathogenesis of atherosclerosis.Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.