Journal of Medical Microbiology 1986-06-01

The detection of leptospires by a chemiluminescent immunoassay.

S A Waitkins, J V Hookey

Index: J. Med. Microbiol. 21(4) , 353-6, (1986)

Full Text: HTML

Abstract

Rabbit serum hyperimmune to Leptospira interrogans var icterohaemorrhagiae serovar icterohaemorrhagiae, reference strain RGA was conjugated to the chemiluminescent label ABEI (6-[N-(4-aminobutyl)-N-ethyl amino]-2,3-dihydrophthalazine-1,4-dione) in the presence of the coupling agent, EDC (1-ethyl-3(3-dimethyl amino-propyl)-carbodiimide HCl). The luminescent conjugate was incubated with homologous and heterologous antigens. The results indicate that chemiluminescence may provide an accurate and rapid method of detecting leptospires in biological fluids.

Related Compounds

  • ABEI

Related Articles:

A label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood.

2016-01-15

[Talanta 147 , 220-5, (2015)]

Development of a solid-phase chemiluminescence immunoassay for plasma progesterone.

1981-07-01

[Steroids 38(1) , 73-88, (1981)]

Enzymatic and kinetic properties of blood coagulation factor XIIIa and guinea pig liver transglutaminase utilizing (6-[N-(4-aminobutyl)-N-ethylamino]-2,3-dihydrophthalazine-1,4-dione, as a novel, specific and sensitive chemiluminescent substrate.

1998-01-01

[J. Biolumin. Chemilumin. 13(1) , 1-11, (1998)]

Chemiluminescent labelled streptavidin (STAV) as a universal marker in steroid and peptide immunoassays.

1989-07-01

[J. Biolumin. Chemilumin. 4(1) , 112-8, (1989)]

Configuration and optimization of a common two-site immunoassay for human prolactin using a chemiluminescent tracer and an enzymatic tracer.

1989-07-01

[J. Biolumin. Chemilumin. 4(1) , 587-93, (1989)]

More Articles...