Biochemical and Biophysical Research Communications 1986-07-16

Covalent binding to apoprotein is a major fate of heme in a variety of reactions in which cytochrome P-450 is destroyed.

F P Guengerich

Index: Biochem. Biophys. Res. Commun. 138(1) , 193-8, (1986)

Full Text: HTML

Abstract

The heme in rat liver microsomal cytochrome P-450 was labeled with 14C or 3H and the microsomes were fractionated after in vitro incubations with a variety of agents known to destroy cytochrome P-450 heme. A major fraction of the heme label was irreversibly bound to apoprotein in all cases, including incubations with fluroxene, 1-octene, vinyl bromide, trichloroethylene, vinyl chloride, parathion, cumene hydroperoxide, NaN3, or iron-ADP complex. Label was also extensively bound to apoprotein when purified and reconstituted cytochrome P-450 was incubated with NADPH and vinyl chloride. This process appears to be widespread and involved to a significant extent in the cytochrome P-450 heme destruction observed with many compounds.

Related Compounds

  • octene

Related Articles:

Characterization of surface confined ionic liquid stationary phases: impact of cation revisited.

2014-10-17

[J. Chromatogr. A. 1364 , 171-82, (2014)]

Rh nanoparticle catalyzed hydroaminomethylation of 1-octene in thermoregulated ionic liquid and organic biphasic system.

2013-07-01

[J. Nanosci. Nanotechnol. 13(7) , 5048-52, (2013)]

Catalytic conversion of bio-oil to oxygen-containing fuels by simultaneous reactions with 1-butanol and 1-octene over solid acids: Model compound studies and reaction pathways.

2013-02-01

[Bioresour. Technol. 130 , 789-92, (2013)]

Isolation and functional analysis of cytochrome P450 CYP153A genes from various environments.

2005-12-01

[Biosci. Biotechnol. Biochem. 69(12) , 2421-30, (2005)]

Photoactivated DNA cleavage by compounds structurally related to the bithiazole moiety of bleomycin.

2001-09-01

[Bioorg. Med. Chem. 9(9) , 2303-14, (2001)]

More Articles...