Immunology Letters 1999-12-01

Expression of an epitopic region of AspfI, an allergen/antigen/cytotoxin of Aspergillus fumigatus.

P V Sarma, S Purkayastha, T Madan, P U Sarma

Index: Immunol. Lett. 70(3) , 151-5, (1999)

Full Text: HTML

Abstract

The gene for an 18 kD allergen/cytotoxin of Aspergillus fumigatus was cloned in pUC-19 vector and expressed in Escherichia coli JM109. Digestion of this gene with AluI resulted in four fragments of 216bp, 120bp, 39bp and 21bp. These fragments were cloned in the Sma-I site of pUC-19. The recombinants thus, generated after transformation in E. coli JM109, were screened using monoclonal antibodies raised against the AspfI. The fusion protein containing 120 bp AluI fragment was recognised by the MoAb indicating presence of epitope(s) in the 120 bp region. The study indicates a viable strategy for identification and expression of an immunologically active domain of a major allergen/antigen of A. fumigatus for the first time.

Related Compounds

  • Restrictocin

Related Articles:

The ribotoxin restrictocin recognizes its RNA substrate by selective engagement of active site residues.

2011-04-12

[Biochemistry 50(14) , 3004-13, (2011)]

Role of individual cysteine residues and disulfide bonds in the structure and function of Aspergillus ribonucleolytic toxin restrictocin.

1999-08-03

[Biochemistry 38(31) , 10052-8, (1999)]

Mechanism of specific target recognition and RNA hydrolysis by ribonucleolytic toxin restrictocin.

2001-08-07

[Biochemistry 40(31) , 9115-24, (2001)]

Electrostatic interactions guide the active site face of a structure-specific ribonuclease to its RNA substrate.

2008-08-26

[Biochemistry 47(34) , 8912-8, (2008)]

[Antibody detection in patients with invasive aspergillosis].

2004-01-01

[Mycoses 47 Suppl 1 , 55-9, (2004)]

More Articles...