Journal of Neuroscience Methods 2011-10-30

Enhancement of neuronal cell adhesion by covalent binding of poly-D-lysine.

Yong Hee Kim, Nam Seob Baek, Young Hwan Han, Myung-Ae Chung, Sang-Don Jung

Index: J. Neurosci. Methods 202 , 38-44, (2011)

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Abstract

We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalent binding between the poly-d-Lysine and substrates and investigated neuronal cell adhesion properties and cell growth morphology. The number of neuronal cell and the number of neurite per neuronal cell on PDL bound surfaces was much more than those on PDL coated surfaces and also the neuronal cells on PDL bounded surfaces survived a longer time. On the pattern of covalently bound PDL, neuronal cells and their neurites are confined within the grid line leading to patterned neuronal networks with the long-term survival.Copyright © 2011 Elsevier B.V. All rights reserved.

Related Compounds

  • Indium Oxide
  • D-Lysine
  • D-Lysine monohydr...

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