Journal of Chromatography B 2012-10-15

Determination of andrograpolide sodium bisulphite in Beagle dog plasma by LC-MS/MS and its application to pharmacokinetics.

Shuang-Qing Zhang, Yu-Ming Fan

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 907 , 173-7, (2012)

Full Text: HTML

Abstract

A sensitive and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the determination of andrographolide sodium bisulphite (ASB) in dog plasma using dehydroandrographolide (DAG) as an internal standard. Chromatographic separation was achieved on a Hypersil Gold C(18) column (50 mm × 2.1 mm, 1.9 μm) with gradient elution that consisted of methanol and water at a flow rate of 0.2 mL/min. Quantification was done using selected reaction monitoring (SRM) mode to monitor precursor-product ion transitions of m/z 413.2→287.2 for ASB and 331.2→303.3 for DAG at negative ionization mode. Good linearity was obtained over the range of 10-1000 ng/mL and the correlation coefficient was better than 0.99. The intra- and inter-day accuracies ranged from 97.2% to 107.8% and precisions (RSD) were within 13.9%. ASB was found stable under three freeze-thaw cycles, short-term temperature, post-preparative and long-term temperature conditions. The method was successfully applied to a pharmacokinetic study of ASB intravenously administered to Beagle dogs.Copyright © 2012 Elsevier B.V. All rights reserved.

Related Compounds

  • Sodium bisulfite
  • Andrographolide

Related Articles:

The high mobility group A2 protein epigenetically silences the Cdh1 gene during epithelial-to-mesenchymal transition.

2015-01-01

[Nucleic Acids Res. 43(1) , 162-78, (2015)]

Purification and inhibition studies with anions and sulfonamides of an α-carbonic anhydrase from the Antarctic seal Leptonychotes weddellii.

2011-03-15

[Bioorg. Med. Chem. 19 , 1847-51, (2011)]

Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR.

2014-01-01

[Nat. Commun. 5 , 5582, (2014)]

Inhibition studies with anions and small molecules of two novel β-carbonic anhydrases from the bacterial pathogenSalmonella entericaserovar Typhimurium

2011-01-01

[Bioorg. Med. Chem. Lett. 21 , 3591-5, (2011)]

Anion inhibition studies of an α-carbonic anhydrase from the living fossil Astrosclera willeyana.

2012-02-01

[Bioorg. Med. Chem. Lett. 22 , 1314-6, (2012)]

More Articles...