Bioanalysis 2013-03-01

Hollow-fiber liquid-phase microextraction and chiral LC-MS/MS analysis of venlafaxine and its metabolites in plasma.

Patrícia da Fonseca, Pierina Sueli Bonato

Index: Bioanalysis 5(6) , 721-30, (2013)

Full Text: HTML

Abstract

An enantioselective analytical method was developed and validated for determination of venlafaxine and its metabolites O-desmethylvenlafaxine and N-desmethylvenlafaxine in plasma samples. The method employed LC-MS/MS analysis and hollow-fiber liquid-phase microextraction (HF LPME) for sample preparation.After HF LPME optimization the following condition was established: sample volume of 4 ml, sample agitation at 1750 rpm, 20 min of extraction, 0.1 mol/l acetic acid as acceptor phase, 1-octanol as organic phase and donor phase pH adjustment to 10. Under these conditions, the method was linear over the concentration range of 5-500 ng/ml with quantification limits of 5 ng/ml.The use of HF LPME for sample preparation provided suitable recoveries, efficient clean-up and low consumption of organic solvent.

Related Compounds

  • 1-Octanol
  • Venlafaxine hydroc...
  • O-Desmethylvenlaf...

Related Articles:

Acetonitrile adduct formation as a sensitive means for simple alcohol detection by LC-MS.

2014-11-01

[J. Am. Soc. Mass Spectrom. 25(11) , 1987-90, (2014)]

Determination of low levels of benzodiazepines and their metabolites in urine by hollow-fiber liquid-phase microextraction (LPME) and gas chromatography-mass spectrometry (GC-MS).

2015-01-15

[J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 975 , 24-33, (2014)]

The EpiOcular Eye Irritation Test (EIT) for hazard identification and labelling of eye irritating chemicals: protocol optimisation for solid materials and the results after extended shipment.

2015-05-01

[Altern. Lab. Anim. 43 , 101-27, (2015)]

Convenient QSAR model for predicting the complexation of structurally diverse compounds with β-cyclodextrins

2009-01-01

[Bioorg. Med. Chem. 17 , 896-904, (2009)]

Evaluation of injection methods for fast, high peak capacity separations with low thermal mass gas chromatography.

2015-05-01

[J. Chromatogr. A. 1392 , 82-90, (2015)]

More Articles...