Biotechnology and Bioengineering 2000-07-05

Dynamic kinetic resolution of suprofen thioester via coupled trioctylamine and lipase catalysis.

C N Lin, S W Tsai

Index: Biotechnol. Bioeng. 69(1) , 31-8, (2000)

Full Text: HTML

Abstract

A lipase-catalyzed enantioselective hydrolysis process under conditions of continuous in situ racemization of substrate with trioctylamine as the catalyst was developed for the production of (S)-suprofen from (R,S)-suprofen 2,2,2-trifluoroethyl thioester in isooctane. A detailed investigation of trioctylamine concentration on the enzyme activation and stability as well as the kinetic behaviors of the thioester in racemization and enzymatic reaction was conducted, in which good agreement between the experimental data and theoretical results was observed. A complete conversion of the racemate for the desired (S)-suprofen in 95% ee(P) was obtained. Moreover, the recovery of the acid product by extraction and reuse of the organic solution were reported.Copyright 2000 John Wiley & Sons, Inc.

Related Compounds

  • Trioctylamine

Related Articles:

Label-Free Nanoplasmonic-Based Short Noncoding RNA Sensing at Attomolar Concentrations Allows for Quantitative and Highly Specific Assay of MicroRNA-10b in Biological Fluids and Circulating Exosomes.

2015-11-24

[ACS Nano 9 , 11075-89, (2015)]

Application of artificial neural network to investigate the effects of 5-fluorouracil on ribonucleotides and deoxyribonucleotides in HepG2 cells.

2015-01-01

[Sci. Rep. 5 , 16861, (2015)]

Hollow fibre liquid phase micro-extraction by facilitated anionic exchange for the determination of flavonoids in faba beans (Vicia faba L.).

2015-01-01

[Phytochem. Anal. 26 , 346-52, (2015)]

Efficient removal of organic ligands from supported nanocrystals by fast thermal annealing enables catalytic studies on well-defined active phases.

2015-06-03

[J. Am. Chem. Soc. 137 , 6906-11, (2015)]

[Analysis of the chemical constituents of essential oil from Chimonanthus zhejiangensis by GC-MS].

2010-03-01

[Zhong Yao Cai 33(3) , 385-7, (2010)]

More Articles...