Journal of Toxicological Sciences 1994-05-01

Acute, subchronic and chronic toxicity studies of a synthetic antioxidant, 2,2'-methylenebis(4-methyl-6-tert-butylphenol) in rats.

A Takagi, K Takada, K Sai, T Ochiai, K Matsumoto, K Sekita, J Momma, Y Aida, M Saitoh, K Naitoh

Index: J. Toxicol. Sci. 19(2) , 77-88, (1994)

Full Text: HTML

Abstract

General toxicity studies on 2,2'-methylenebis(4-methyl-6-tert-butylphenol) (MBMBP) were conducted using male and female Wistar rats. LD50 values were greater than 5 g/kg BW by oral administration for both sexes. Diarrhea was observed until 5 days. In the subchronic test, rats were fed diet containing MBMBP at 0, 0.12, 0.6 or 3.0% for 12 weeks. Severe suppression of body weight gain was observed in both sexes of 0.6 and 3.0% groups. Death accompanied by hemorrhage from nasal cavity was observed in 0.6 and 3.0% males and 3.0% females. Dose-dependent toxicity to the liver in both sexes was observed in blood chemical analysis. Histopathologically, testicular atrophy and decrease of spermatogenesis were dose- and time-dependently observed in all treated males. Atrophy of ovaries was evident in 0.6 and 3.0% females. Thymus atrophy and bone marrow hypoplasia were observed in both sexes of 0.6 and 3.0% groups. In the chronic test, rats were fed diet containing MBMBP at 0, 0.01, 0.03 and 0.1% for 18 months. Body weight gain was only suppressed in both sexes receiving 0.1%. Histopathologically, testicular atrophy and decrease of spermatogenesis were apparent in 0.1% males. No neoplastic response by MBMBP administration was noted. NOAEL was concluded to be 0.03% in the diet (12.7 mg/kg BW/day for male rats and 15.1 mg/kg BW/day for female rats).


Related Compounds

  • 2,2'-Methylenebis(...

Related Articles:

Bis(2-hydroxy-3-tert-butyl-5-methyl-phenyl)-methane (bis-phenol) is a potent and selective inhibitor of the secretory pathway Ca²⁺ ATPase (SPCA1).

2012-08-03

[Biochem. Biophys. Res. Commun. 424(3) , 616-9, (2012)]

Effects of four bisphenolic antioxidants on lipid contents of rat liver.

1981-04-01

[Toxicol. Lett. 8(1-2) , 77-86, (1981)]

Inhibition of the sarcoplasmic and endoplasmic calcium pump by bis(2-hydroxy-3-tert-butyl-5-methyl)methane, (bis-phenol).

1993-05-01

[Biochem. Soc. Trans. 21(2) , 106S, (1993)]

[Studies on the teratogenic potential of 2,2'-methylenebis (4-methyl-6-tert-butylphenol) in rats].

1990-01-01

[Eisei Shikenjo Hokoku. (108) , 52-7, (1990)]

Characterisation of a novel Ca2+ pump inhibitor (bis-phenol) and its effects on intracellular Ca2+ mobilization.

1994-11-02

[Biochim. Biophys. Acta 1195(2) , 252-8, (1994)]

More Articles...