Gene 1979-05-01

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

M Dagert, S D Ehrlich

Index: Gene 6(1) , 23-8, (1979)

Full Text: HTML

Abstract

Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment. With 24-h-old competent cells we obtained routinely 2 . 10(7) transformants per microgram of pBR322 DNA, and transformed over 20% of viable cells.

Related Compounds

  • Calcium chloride h...
  • calcium chloride d...

Related Articles:

Selective removal of phosphate for analysis of organic acids in complex samples.

2015-04-03

[J. Chromatogr. A. 1388 , 1-8, (2015)]

Stability of peptide drugs in the colon.

2015-10-12

[Eur. J. Pharm. Sci. 78 , 31-6, (2015)]

On the colonic bacterial metabolism of azo-bonded prodrugsof 5-aminosalicylic acid.

2014-10-01

[J. Pharm. Sci. 103(10) , 3171-5, (2014)]

Improved sensitivity of the nano ultra-high performance liquid chromatography-tandem mass spectrometric analysis of low-concentrated neuropeptides by reducing aspecific adsorption and optimizing the injection solvent.

2014-09-19

[J. Chromatogr. A. 1360 , 217-28, (2014)]

Data mining crystallization databases: knowledge-based approaches to optimize protein crystal screens.

2003-06-01

[Proteins 51(4) , 562-8, (2003)]

More Articles...