Determination of homovanillic, isohomovanillic and vanillylmandelic acids in human urine by means of glass capillary gas-liquid chromatography with temperature-programmed electron-capture detection.

Ulrike E Hille, Qingzhong Hu, Carsten Vock, Matthias Negri, Marc Bartels, Ursula Müller-Vieira, Thomas Lauterbach, Rolf W Hartmann

Index: J. Chromatogr. A. 183(4) , 391-401, (1980)

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Abstract

The determination of urinary homovanillic, isohomovanillic and vanillylmandelic acids as their trifluoroacetylhexafluoroisopropyl ester derivatives by glass capillary gas-liquid chromatography has been studied. It was shown that even with high column efficiencies a single peak-single compound relationship could not be assumed and for reliable quantitation it was necessary to check determinations with a second gas-liquid chromatography column.

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