Amino Acids 2012-11-01

Amperometric microbiosensor as an alternative tool for investigation of D-serine in brain.

Zainiharyati Mohd Zain, Sulaiman Ab Ghani, Robert D O'Neill

Index: Amino Acids 43(5) , 1887-94, (2012)

Full Text: HTML

Abstract

This paper discusses the application of a reagentless, selective microbiosensor as a useful alternative tool for monitoring D-serine in neural samples. The main components of the 125-μm-diameter disk biosensor were D-amino acid oxidase for D-serine sensitivity (linear region slope, 61 ± 7 μA cm(-2) mM(-1); limit of detection, 20 nM), and poly-phenylenediamine for rejection of electroactive interference. The response time of the biosensor was of the order of 1 s, ideal for 'real-time' monitoring, and detection of systemically administered D-serine in brain extracellular fluid is demonstrated. Exploitation of this probe might resolve queries involving regulation of D-serine in excitotoxicity, and modulation of N-methyl-D-aspartate receptor function by D-serine and glycine in the central nervous system.

Related Compounds

  • L-serine
  • D-Amino acid oxid...

Related Articles:

Long-term application of glycine transporter inhibitors acts antineuropathic and modulates spinal N-methyl-D-aspartate receptor subunit NR-1 expression in rats.

2014-07-01

[Anesthesiology 121(1) , 160-9, (2014)]

8DSS-promoted remineralization of initial enamel caries in vitro.

2014-05-01

[J. Dent. Res. 93(5) , 520-4, (2014)]

Sulfur amino acid metabolism in Zucker diabetic fatty rats.

2015-08-01

[Biochem. Pharmacol. 96 , 256-66, (2015)]

Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli.

2015-01-01

[BMC Genomics 16 , 1051, (2015)]

Use of Commercial Dry Yeast Products Rich in Mannoproteins for White and Rosé Sparkling Wine Elaboration.

2015-06-17

[J. Agric. Food Chem. 63 , 5670-81, (2015)]

More Articles...