Toxicon 1988-01-01

Strongly enhanced toxicity of the mushroom toxin alpha-amanitin by an amatoxin-specific Fab or monoclonal antibody.

H Faulstich, K Kirchner, M Derenzini

Index: Toxicon 26(5) , 491-9, (1988)

Full Text: HTML

Abstract

A monoclonal antibody, with high affinity against the mushroom toxin alpha-amanitin, was prepared. Administration of the Fab fragment of the monoclonal antibody to mice caused a 50-fold increase in alpha-amanitin toxicity. Electron micrographs showed normal appearance of hepatocytes but typical, amanitin-induced lesions in cells of the proximal convoluted tubules of the kidney. The pronounced nephrotoxicity is mainly explained by glomerular filtration and tubular reabsorption of the Fab-amatoxin complex and, to a lesser extent, of the immunoglobulin-amatoxin complex, which is still c. Twice as toxic as free alpha-amanitin. To our knowledge this is the first reported case where immunoglobulins or their fragments enhance rather than decrease the activity of a toxin. Accordingly, immunotherapy of Amanita mushroom poisoning in humans does not appear promising.

Related Compounds

  • beta-Amanitin

Related Articles:

[Determination of amanitatoxins by HPLC].

2003-10-01

[Chudoku. Kenkyu. 16(4) , 441-5, (2003)]

Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) in toxicological analysis. Studies on the detection of clobenzorex and its metabolites within a systematic toxicological analysis procedure by GC-MS and by immunoassay and studies on the detection of alpha- and beta-amanitin in urine by atmospheric pressure ionization electrospray LC-MS.

1997-02-07

[J. Chromatogr. B. Biomed. Sci. Appl. 689(1) , 81-9, (1997)]

Validated electrospray liquid chromatographic-mass spectrometric assay for the determination of the mushroom toxins alpha- and beta-amanitin in urine after immunoaffinity extraction.

2000-10-01

[J. Chromatogr. B. Biomed. Sci. Appl. 748(1) , 125-35, (2000)]

Determination of alpha-amanitin and beta-amanitin in human biological fluids by high-performance liquid chromatography.

1985-08-15

[Anal. Biochem. 149(1) , 35-42, (1985)]

Determination of alpha- and beta-amanitin in clinical urine samples by Capillary Zone Electrophoresis.

2008-08-05

[J. Pharm. Biomed. Anal. 47(4-5) , 913-7, (2008)]

More Articles...