Analytical Sciences 2016-01-01

The Use of an Enzyme-based Sensor Array to Fingerprint Proteomic Signatures of Sera from Different Mammalian Species.

Shunsuke Tomita, Saki Yokoyama, Ryoji Kurita, Osamu Niwa, Keitaro Yoshimoto

Index: Anal. Sci. 32 , 237-40, (2016)

Full Text: HTML

Abstract

A cross-reactive sensor array consisting of polyion complexes (PICs) between anionic enzymes and poly(ethylene glycol)-modified (PEGylated) polyamines has been used to identify the source of mammalian sera. Although the catalytic activity of enzymes was inhibited by PIC formation with PEGylated polyamines, the subsequent addition of sera caused enzyme releases from PICs through competitive interactions between PICs and serum proteins, generating unique response patterns of changes in the enzyme activity. Linear discriminant analysis of the obtained patterns enabled the discrimination of five sera from different mammalian sources.

Related Compounds

  • Triton X-100
  • 4-Methylumbellifer...

Related Articles:

A survey of the interactome of Kaposi's sarcoma-associated herpesvirus ORF45 revealed its binding to viral ORF33 and cellular USP7, resulting in stabilization of ORF33 that is required for production of progeny viruses.

2015-05-01

[J. Virol. 89(9) , 4918-31, (2015)]

Phospho-tyrosine dependent protein-protein interaction network.

2015-03-01

[Mol. Syst. Biol. 11(3) , 794, (2015)]

The combination of electric current and copper promotes neuronal differentiation of adipose-derived stem cells.

2015-04-01

[Ann. Biomed. Eng. 43(4) , 1014-23, (2015)]

A precisely substituted benzopyran targets androgen refractory prostate cancer cells through selective modulation of estrogen receptors.

2015-03-15

[Toxicol. Appl. Pharmacol. 283(3) , 187-97, (2015)]

Involvement of epigenetics and EMT-related miRNA in arsenic-induced neoplastic transformation and their potential clinical use.

2015-03-01

[Cancer Prev. Res. (Phila.) 8(3) , 208-21, (2015)]

More Articles...