Alimentary Pharmacology and Therapeutics 1996-06-01

How accurate is the determination of blood in gastric juice? Comparison of peroxidase and porphyrin methods.

K L Woods, C Kiefe, D Y Graham

Index: Aliment. Pharmacol. Ther. 10(3) , 333-7, (1996)

Full Text: HTML

Abstract

Mucosal bleeding is frequently used as a measure of gastric mucosal integrity. We compared the orthotolidine method for quantifying haemoglobin in gastric juice with a method based on measurement of fluorescent porphyrins extracted from haem. We also investigated whether acid or pepsin had a deleterious effect on the results of either method.We compared the effects of pH (2 or 7), time (up to 180 min), haemoglobin concentration and the addition of pepsin (2.5, 5 or 7.5 kU/mL hog pepsin) on the accuracy of the two methods.With the orthotolidine method there was a time-dependent decline in detectable haemoglobin concentration (P < 0.02) at pH 2 that was not seen at pH 7. The time- and pH-dependent decline in detectable haemoglobin was not seen using the porphyrin assay.The widely used orthotolidine method for determination of blood in gastric juice is less reliable than the porphyrin method as it is more likely to be influenced by acidic conditions in the stomach.

Related Compounds

  • 3,3'-dimethylbenzi...
  • 4,4'-Bi-o-toluidin...

Related Articles:

Evidence that 4-aminobiphenyl, benzidine, and benzidine congeners produce genotoxicity through reactive oxygen species.

2007-06-01

[Environ. Mol. Mutagen. 48(5) , 404-13, (2007)]

Biopartitioning micellar chromatography to predict mutagenicity of aromatic amines.

2007-01-01

[Eur. J. Med. Chem. 42 , 1396-402, (2007)]

ortho-Substituent effects on the in vitro and in vivo genotoxicity of benzidine derivatives.

1993-09-01

[Mutat. Res. 319(1) , 19-30, (1993)]

Iron(III) oxidized nucleophilic coupling of catechol with o-tolidine/p-toluidine followed by 1,10-phenanthroline as new and sensitivity improved spectrophotometric methods for iron present in chemicals, pharmaceutical, edible green leaves, nuts and lake water samples.

2012-02-01

[Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 86 , 152-8, (2012)]

Electronic, infrared, and 1HNMR spectral studies of the novel charge-transfer complexes of o-tolidine and p-toluidine with alternation pi-acceptors (3,5-dinitro benzoic acid and 2,6-dichloroquinone-4-chloroimide) in CHCl3 solvent.

2006-06-01

[Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 64(3) , 778-88, (2006)]

More Articles...