Carbohydrate Research 2001-01-30

Studies toward the site specific incorporation of sugars into proteins: synthesis of glycosylated aminoacyl-tRNAs.

N E Fahmi, S Golovine, B Wang, S M Hecht

Index: Carbohydr. Res. 330(2) , 149-64, (2001)

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Abstract

A series of glycosylated serine derivatives was synthesized from peracetylated sugars and Fmoc-protected serine; these were chemically esterified with the tris-(tetrabutylammonium) salt of pdCpA. The fully protected and deprotected glycosylated aminoacyl pdCpAs were ligated enzymatically to an abbreviated tRNA (tRNA-C(OH)) to provide the title compounds that are key intermediates in the elaboration of glycoproteins using readthrough of a nonsense codon.

Related Compounds

  • 5-(Dimethylamino)-...

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