Developments in biological standardization 1981-01-01

Control on the absence of DEAE-polysaccharides in DEAE-sephadex purified poliovirus suspensions by pyrolysis mass spectrometry.

W Windig, J Haverkamp, A L van Wezel

Index: Dev. Biol. Stand. 47 , 169-77, (1981)

Full Text: HTML

Abstract

Poliovirus suspensions produced in microcarrier culture of primary monkey kidney cells and purified by column chromatography on DEAE-sephadex have been tested for the presence of DEAE-polysaccharides (in essence DEAE-dextran) by pyrolysis mass spectrometry. Semi-quantitative evaluation of the pyrolysis mass spectra indicates that the concentration of DEAE-dextran in the purified poliovirus concentrates is below 20 ppm i.e. the lower detection level of the method. As the final vaccines contain a 1:50 to 1:100 dilution of the tested virus suspension, the DEAE-polysaccharide concentration in the vaccine will be below 1 ppm at least. Pyrolysis mass spectrometry appeared to be a vary suitable method for control on the absence of this component.

Related Compounds

  • DEAE_Sephadex

Related Articles:

Myoglobin oxygen affinity in aquatic and terrestrial birds and mammals.

2015-07-01

[J. Exp. Biol. 218 , 2180-9, (2015)]

A monovalent anion affected multi-functional cellulase EGX from the mollusca, Ampullaria crossean.

2003-09-01

[Protein Expr. Purif. 31(1) , 108-114, (2003)]

[Use of DEAE-Sephadex A-50 microcarriers for reproduction of the mumps virus].

1984-01-01

[Vopr. Virusol. 29(1) , 51-5, (1984)]

[Preparation of a prothrombin complex concentrate (use of DEAE-cellulose and DEAE-sephadex)].

1985-06-01

[Vnitr. Lek. 31(6) , 602-7, (1985)]

Immune globulin subcutaneous (human) vivaglobin: for the treatment of primary immunodeficiency diseases.

2009-06-01

[P T 34(6) , 2-21, (2009)]

More Articles...