PLoS ONE 2015-01-01

The Energy of COPI for Budding Membranes.

Abdou Rachid Thiam, Frédéric Pincet

Index: PLoS ONE 10 , e0133757, (2015)

Full Text: HTML

Abstract

As a major actor of cellular trafficking, COPI coat proteins assemble on membranes and locally bend them to bud 60 nm-size coated particles. Budding requires the energy of the coat assembly to overcome the one necessary to deform the membrane which primarily depends on the bending modulus and surface tension, γ. Using a COPI-induced oil nanodroplet formation approach, we modulated the budding of nanodroplets using various amounts and types of surfactant. We found a Heaviside-like dependence between the budding efficiency and γ: budding was only dependent on γ and occurred beneath 1.3 mN/m. With the sole contribution of γ to the membrane deformation energy, we assessed that COPI supplies ~1500 kBT for budding particles from membranes, which is consistent with common membrane deformation energies. Our results highlight how a simple remodeling of the composition of membranes could mechanically modulate budding in cells.

Related Compounds

  • oleic acid
  • Glyceryl Dioleate

Related Articles:

Differential expression of efflux pump genes of Mycobacterium tuberculosis in response to varied subinhibitory concentrations of antituberculosis agents.

2015-03-01

[Tuberculosis (Edinb.) 95(2) , 155-61, (2015)]

Decreased lipogenesis in white adipose tissue contributes to the resistance to high fat diet-induced obesity in phosphatidylethanolamine N-methyltransferase-deficient mice.

2014-10-01

[Biochim. Biophys. Acta 1851(2) , 152-62, (2015)]

Exploring the impact of drug properties on the extent of intestinal lymphatic transport - in vitro and in vivo studies.

2015-05-01

[Pharm. Res. 32(5) , 1817-29, (2015)]

Sensing of triacylglycerol in the gut: different mechanisms for fatty acids and 2-monoacylglycerol.

2015-04-15

[J. Physiol. 593(8) , 2097-109, (2015)]

Mouse aldehyde dehydrogenase ALDH3B2 is localized to lipid droplets via two C-terminal tryptophan residues and lipid modification.

2015-01-01

[Biochem. J. 465(1) , 79-87, (2015)]

More Articles...