Free Radical Biology and Medicine 1994-10-01

Methoxatin (PQQ) in guinea-pig neutrophils.

A Bishop, M A Paz, P M Gallop, M L Karnovsky

Index: Free Radic. Biol. Med. 17(4) , 311-20, (1994)

Full Text: HTML

Abstract

PQQ, also called methoxatin, has been isolated from guinea-pig neutrophils. The organic cations diphenyleneiodonium (DPI) and diphenyliodonium (BPI) and the aromatic o-diamine 4,5-dimethylphenylenediamine (DIMPDA) sequester synthetic PQQ and inhibit its redox-cycling activity in a model system. Standards were made of adducts of tritiated PQQ with unlabeled DIMPDA and of unlabeled PQQ with tritiated DPI or DIMPDA. PQQ adducts were isolated from guinea-pig neutrophils with each of the tritiated inhibitors. They were separated and defined by high-performance liquid chromatography (HPLC). Tiron, a disodium benzene disulphonic acid, broke the DPI-PQQ adduct isolated from neutrophils and released free PQQ. Both DPI and DIMPDA, as well as BPI, blocked O2.- release by stimulated neutrophils. The blockade exerted by these inhibitors was released by the addition of PQQ to the cell suspensions. The data demonstrate the presence of PQQ in guinea-pig neutrophils and suggest that it has a possible role, direct or indirect, in the O2.(-)-producing respiratory burst.

Related Compounds

  • 2-amino-4,5-dimeth...

Related Articles:

The acute phase protein ceruloplasmin as a non-invasive marker of pseudopregnancy, pregnancy, and pregnancy loss in the giant panda.

2011-01-01

[PLoS ONE 6(7) , e21159, (2011)]

Synthesis of new derivatives of 4-acylquinoxalin-2-ones with potential pharmacological activity.

1994-01-01

[Acta Pol. Pharm. 51 , 231, (1994)]

Ruthenium(II) arene anticancer complexes with redox-active diamine ligands.

2009-10-05

[Inorg. Chem. 48(19) , 9444-53, (2009)]

Development and validation of a liquid chromatographic method for the determination of ascorbic acid, dehydroascorbic acid and acetaminophen in pharmaceuticals.

2008-09-29

[J. Pharm. Biomed. Anal. 48(2) , 331-9, (2008)]

Aspects of the release of superoxide by leukocytes, and a means by which this is switched off.

1994-12-01

[Environ. Health Perspect. 102 Suppl 10 , 43-4, (1994)]

More Articles...