Journal of Biochemistry 1977-11-01

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.

T Morita, H Kato, S Iwanaga, K Takada, T Kimura

Index: J. Biochem. Tokyo 82 , 1495, (1977)

Full Text: HTML

Abstract

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were found to be specific substrates for individual enzymes: Boc-Val-Pro-Arg-MCA for alpha-thrombin, Boc-Ile-Glu-Gly-Arg-MCA, and Boc-Ser-Gly-Arg-MCA for factor Xa, Z-Phe-Arg-MCA for plasma kallikrein, Pro-Phe-Arg-MCA for pancreatic and urinary kallikreins, and glutaryl-Gly-Arg-MCA for urokinase. Moreover, these peptide-MCA substrates were resistant to plasmin.

Related Compounds

  • Z-Phe-Arg-AMC ·...
  • H-Pro-Phe-Arg-A...
  • Z-Gly-Pro-Leu-Gl...
  • boc-val-pro-arg-am...

Related Articles:

Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates.

1981-01-01

[Meth. Enzymol. 80 , 341, (1981)]

Residue-specific annotation of disorder-to-order transition and cathepsin inhibition of a propeptide-like crammer from D. melanogaster.

2013-01-01

[PLoS ONE 8(1) , e54187, (2013)]

Production of anti-peptide antibodies against trypanopain-Tb from Trypanosoma brucei brucei: effects of antibodies on enzyme activity against Z-Phe-Arg-AMC.

1997-06-01

[Immunopharmacology 36(2-3) , 295-303, (1997)]

Modulation of the catalytic activity of cruzipain, the major cysteine proteinase from Trypanosoma cruzi, by temperature and pH.

2001-06-01

[Eur. J. Biochem. 268(11) , 3253-8, (2001)]

Delineating functionally important regions and residues in the cathepsin B propeptide for inhibitory activity.

1996-09-09

[FEBS Lett. 393(1) , 24-6, (1996)]

More Articles...