Bioinorganic Chemistry and Applications 2011-01-01

Identification of Binding Mode of a Platinum (II) Complex, PtCl(2)(DIP), and Calf Thymus DNA.

Nahid Shahabadi, Soheila Kashanian, Azadeh Fatahi

Index: Bioinorg. Chem. Appl. 2011 , 687571, (2011)

Full Text: HTML

Abstract

The Pt(II) complex, PtCl(2)(DIP) (DIP = chelating dinitrogen ligand: 4,7-diphenyl-1,10-phenanthroline), was synthesized and characterized by elemental analysis (CHN) and (1)H NMR and UV-vis techniques. The binding of this complex to calf thymus DNA was investigated using various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hyperchromism) of calf thymus DNA (CT-DNA): increase in melting temperature, sharp increase in specific viscosity of DNA, and induced CD spectral changes. Also the fluorescence spectral characteristics and interaction of Pt complex with DNA have been studied. Pt bound to DNA showed a marked decrease in the fluorescence intensity. The results show that both the complex and the NR molecules can intercalate competitively into the DNA double-helix structure. The experimental results show that the mode of binding of the this complex to DNA is classical intercalation.


Related Compounds

  • 3-(Trimethylsilyl)...

Related Articles:

A metabolomic study of adipose tissue in mice with a disruption of the circadian system.

2015-07-01

[Mol. Biosyst. 11 , 1897-906, (2015)]

Ethanol contamination of cerebrospinal fluid during standardized sampling and its effect on (1)H-NMR metabolomics.

2015-06-01

[Anal. Bioanal. Chem 407 , 4835-9, (2015)]

Water Proton NMR for In Situ Detection of Insulin Aggregates.

2015-12-01

[J. Pharm. Sci. 104 , 4132-41, (2016)]

The antitumor effect of formosanin C on HepG2 cell as revealed by 1H-NMR based metabolic profiling.

2014-09-05

[Chem. Biol. Interact. 220 , 193-9, (2014)]

Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women--a randomized control trial.

2012-01-01

[Nutr. J. 11 , 88, (2012)]

More Articles...