Medical Microbiology and Immunology 2007-03-01

Streptococcus agalactiae CAMP factor binds to GPI-anchored proteins.

Shenhui Lang, Jie Xue, Zhongwu Guo, Michael Palmer

Index: Med. Microbiol. Immunol. 196(1) , 1-10, (2007)

Full Text: HTML

Abstract

CAMP factor (protein B) is a pore-forming protein secreted by Streptococcus agalactiae. It causes lysis of sheep red blood cells when these have been sensitized with staphylococcal sphingomyelinase. We here show that CAMP factor binds to GPI-anchored proteins, and that this interaction involves the carbohydrate core of the GPI-anchor. Enzymatic cleavage of GPI-anchors with phosphatidylinositol-specific phospholipase C strongly reduces the sensitivity of erythrocytes to CAMP factor. Incorporation of alkaline phosphatase, a model GPI-anchored protein, into liposome membranes renders the latter susceptible to permeabilization by CAMP factor. GPI-anchored proteins therefore function as cellular receptors for CAMP factor.

Related Compounds

  • PI-PLC,1-Phosph...

Related Articles:

Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.

2010-12-31

[J. Biol. Chem. 285 , 41765-71, (2010)]

Francisella targets cholesterol-rich host cell membrane domains for entry into macrophages.

2008-06-15

[J. Histochem. Cytochem. 180 , 8262-71, (2008)]

Roles of brain phosphatidylinositol-specific phospholipase C and diacylglycerol lipase in centrally administered histamine-induced adrenomedullary outflow in rats.

2007-10-01

[Eur. J. Pharmacol. 571(2-3) , 138-44, (2007)]

Monophosphatidyl inositol inositolphosphohydrolase in guinea-pig brain.

1967-12-05

[Biochim. Biophys. Acta 144(3) , 684-6, (1967)]

Cell signaling. The art of the soluble.

2007-05-11

[Science 316(5826) , 845-6, (2007)]

More Articles...