Applied and Environmental Microbiology 2012-04-01

Cloning and sequencing of inulinase and β-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes.

Tohru Kobayashi, Kohsuke Uchimura, Shigeru Deguchi, Koki Horikoshi

Index: Appl. Environ. Microbiol. 78(7) , 2493-5, (2012)

Full Text: HTML

Abstract

An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and the other was a β-fructofuranosidase. Both enzymes worked together to effectively degrade inulin.

Related Compounds

  • Inulinase fromAsp...

Related Articles:

Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp.

2013-11-01

[Bioresour. Technol. 147 , 254-9, (2013)]

Molecular characterization and expression of microbial inulinase genes.

2013-05-01

[Crit. Rev. Microbiol 39(2) , 152-65, (2013)]

First crystal structure of an endo-inulinase, INU2, from Aspergillus ficuum: discovery of an extra-pocket in the catalytic domain responsible for its endo-activity.

2012-11-01

[Biochimie 94(11) , 2423-30, (2012)]

Consolidated bioprocessing of highly concentrated Jerusalem artichoke tubers for simultaneous saccharification and ethanol fermentation.

2013-10-01

[Biotechnol. Bioeng. 110(10) , 2606-15, (2013)]

Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast.

2011-08-01

[Appl. Microbiol. Biotechnol. 91(3) , 789-98, (2011)]

More Articles...