Journal of Bioscience and Bioengineering 2005-09-01

Hyper expression of kojibiose phosphorylase gene and trehalose phosphorylase gene from Thermoanaerobacter brockii ATCC35047 in Bacillus subtilis and selaginose synthesis utilizing two phosphorylases.

Takuo Yamamoto, Kazuhisa Mukai, Kazuhiko Maruta, Hikaru Watanabe, Hiroshi Yamashita, Tomoyuki Nishimoto, Michio Kubota, Hiroto Chaen, Shigeharu Fukuda

Index: J. Biosci. Bioeng. 100(3) , 343-6, (2005)

Full Text: HTML

Abstract

The kojibiose phosphorylase (KP) gene and trehalose phosphorylase (TP) gene from Thermoanaerobacter brockii ATCC35047 were intracellularly hyper-expressed under the control of the Bacillus amyloliquefaciens alpha-amylase promoter in Bacillus subtilis. The production yields were estimated to be 2.1 g of KP and 4.9 g of TP per liter of medium. Selaginose, non-reducing trisaccharide, was synthesized from trehalose utilizing the recombinant KP and TP from B. subtilis. Selaginose was not hydrolyzed by salivary amylase, artificial gastric juice, pancreatic amylase, or small intestinal enzymes.

Related Compounds

  • Kojibiose

Related Articles:

Extracellular and cell-associated forms of Gluconobacter oxydans dextran dextrinase change their localization depending on the cell growth.

2015-01-02

[Biochem. Biophys. Res. Commun. 456(1) , 500-5, (2015)]

Differentiation of Disaccharide Isomers by Temperature-Dependent In-Source Decay (TDISD) and DART-Q-TOF MS/MS.

2015-09-01

[J. Am. Soc. Mass Spectrom. 26 , 1599-605, (2015)]

Microbial Communities Can Be Described by Metabolic Structure: A General Framework and Application to a Seasonally Variable, Depth-Stratified Microbial Community from the Coastal West Antarctic Peninsula.

2015-01-01

[PLoS ONE 10 , e0135868, (2015)]

Transfer of oligosaccharide from oligosaccharide pyrophosphoryl dolichol to endogenous acceptor proteins in human breast malignant and normal tissues.

1985-07-15

[Experientia 41(7) , 928-9, (1985)]

Enzymatic synthesis of a 2-O-alpha-D-glucopyranosyl cyclic tetrasaccharide by kojibiose phosphorylase.

2005-02-28

[Carbohydr. Res. 340(3) , 449-54, (2005)]

More Articles...