Spectrophotometric and spectrofluorimetric methods for determination of Racecadotril.

Nouruddin Wageih Ali, Mohammed Refaat Elghobashy, Mohammed Gamal Mahmoud, Mohammed Abdelkawy Mohammed

Index: Pak. J. Pharm. Sci. 24(1) , 19-23, (2011)

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Abstract

Two accurate and sensitive spectrophotometric and spectrofluorimetric methods were developed for determination of Racecadotril. In the first method reduction of Fe3+ into Fe2+ in presence of o-phenanthroline by Racecadotril to form a stable orange-red ferroin chelate [Fe-(Phen)3]2+ was the basis for its determination. The absorbance at 510 nm was measured and linear correlation was obtained in the concentration range of 2.5-25 µg mL(-1). In the second method the native fluorescence of Racecadotril in acetonitrile solvent at λ=319 nm when excitation was at 252 nm is used for its determination. Linear correlation was obtained in the concentration range of 50 to 500 ng mL(-1). The proposed methods were applied for determination of Racecadotril in bulk powder with mean accuracy of 100.39±1.239 for the spectrophotometric method and 100.09±1.042 for the spectrofluorimetric method. The proposed methods were successfully applied for determination of Racecadotril in its pharmaceutical dosage form.