Simultaneous determination of quinolones for veterinary use by high-performance liquid chromatography with electrochemical detection.

M I Rodríguez Cáceres, A Guiberteau Cabanillas, T Galeano Díaz, M A Martínez Cañas

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 878(3-4) , 398-402, (2010)

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Abstract

A selective method based on high-performance liquid chromatography with electrochemical detection (HPLC-ECD) has been developed to enable simultaneous determination of three fluoroquinolones (FQs), namely danofloxacin (DANO), difloxacin (DIFLO) and sarafloxacin (SARA). The fluoroquinolones are separated on a Novapack C-18 column and detected in a high sensitivity amperometric cell at a potential of +0.8 V. Solid-phase extraction was used for the extraction of the analytes in real samples. The range of concentration examined varied from 10 to 150 ng g(-1) for danofloxacin, from 25 to 100 ng g(-1) for sarafloxacin and from 50 to 315 ng g(-1) for difloxacin, respectively. The method presents detection limits under 10 ng g(-1) and recoveries around 90% for the three analytes have been obtained in the experiments with fortified samples. This HPLC-ECD approach can be useful in the routine analysis of antibacterial residues being less expensive and less complicated than other more powerful tools as hyphenated techniques.2009 Elsevier B.V. All rights reserved.