Scientific reports 2012-01-01

Photo-induced proton gradients for the in vitro investigation of bacterial efflux pumps.

Alice Verchère, Isabelle Broutin, Martin Picard

Index: Sci. Rep. 2 , 306, (2012)

Full Text: HTML

Abstract

We describe an original activity assay for membrane transport that uses the proton motive force-dependent efflux pump MexAB from Pseudomonas aeruginosa. This pump is co-reconstituted into proteoliposomes together with bacteriorhodopsin (BR), a light-activated proton pump. In this system, upon illumination with visible light, the photo-induced proton gradient created by the BR is shown to be coupled to the active transport of substrates through the pump.

Related Compounds

  • Spautin-1

Related Articles:

Single Chain Variable Fragments Produced in Escherichia coli against Heat-Labile and Heat-Stable Toxins from Enterotoxigenic E. coli.

2015-01-01

[PLoS ONE 10 , e0131484, (2015)]

Photosynthetic production of enantioselective biocatalysts.

2015-01-01

[Microb. Cell Fact. 14 , 53, (2015)]

Outer-membrane translocation of bulky small molecules by passive diffusion.

2015-06-09

[Proc. Natl. Acad. Sci. U. S. A. 112 , E2991-9, (2015)]

Effects of pre- and postnatal exposure to flutamide on connexin 43 expression in testes and ovaries of prepubertal pigs.

2010-01-01

[Eur. J. Histochem. 54(2) , e15, (2010)]

Cellulose Microfibril Formation by Surface-Tethered Cellulose Synthase Enzymes.

2016-02-23

[ACS Nano 10 , 1896-907, (2016)]

More Articles...