Effects of some chelating agents on the uptake and distribution of 54Mn(II) in the brown trout (Salmo trutta).

C Rouleau, H Tjälve, J Gottofrey, E Pelletier

Index: Pharmacol. Toxicol. 74(4-5) , 271-9, (1994)

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Abstract

The effects of humic acids, which are natural metal-complexing compounds, and potassium ethylxanthate, sodium diethyldithiophosphate, sodium dimethyldithiocarbamate, and sodium diethyldithiocarbamate, which are sulphur-containing man-made chelating agents, on the uptake and tissue distribution of 54Mn(II) were studied in brown trout (Salmo trutta). Fish were exposed for 7 days to 0.1 microgram Mn(II).l-1 as MnCl2 (1 microCi 54MN.l-1) with or without chelating agents. Examination of the partition of Mn between octanol and a Tris-HCl buffer in the presence of these compounds was also performed. Humic acids had only small effects on Mn uptake and distribution in trout, probably because of the low stability of Mn-humate complexes. Partition of Mn in the presence of potassium ethylxanthate, sodium diethyldithiophosphate, sodium dimethyldithiocarbamate, and sodium diethyldithiocarbamate between octanol and Tris-HCl buffer showed formation of lipophilic complex with the latter two compounds, but not with the former. However, these four chelating agents all decreased Mn uptake in the trout by 40-45%. These substances also changed the distribution of Mn within the fish, with a higher proportion of the metal being present in some visceral organs and a smaller proportion being localized in some non-parenchymateous tissues, such as skins, fins and bones. The mechanisms underlying these effects are not known. However, the interaction of chelating agents with the Mn, although weak, may have partially withdrawn the metal from the uptake process in the gills. The redistribution of Mn in the fish may be due to the binding of the metal to complexing compounds which have reached the intestinal lumen.(ABSTRACT TRUNCATED AT 250 WORDS)