Enantioselective synthesis of (S)-amino acids by phenylalanine dehydrogenase from Bacillus sphaericus: use of natural and recombinant enzymes

Y Asano, A Yamada, Y Kato, K Yamaguchi…

Index: Asano; Yamada; Kato; Yamaguchi; Hibino; Hirai; Kondo Journal of Organic Chemistry, 1990 , vol. 55, # 21 p. 5567 - 5571

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Citation Number: 90

Abstract

The substrate specificity of phenylalanine dehydrogenase (L-phenylalanine: NAD+ oxidoreductase, deaminating (EC 1.4. 1.-), PheDH) from Bacillus sphaericus SCRC-R79a in the reductive amination reaction was examined with various natural and synthetic 2-and% OXO acids and their analogues. The enzyme was active toward 3-substituted pyruvic acids with bulky substituents. Optically pure (S)-phenylalanine and other (S)-amino acids were ...