Journal of AOAC International 2013-01-01

Multivariate optimization of separation conditions for simultaneous determination of acemetacin and chlorzoxazone in a pharmaceutical preparation by HPLC using response surface methodology.

Cigdem Aybaba, Ismail Murat Palabiyik, Mehmet Gokhan Caglayan, Feyyaz Onur

Index: J. AOAC Int. 96(4) , 723-9, (2013)

Full Text: HTML

Abstract

A new, fast, accurate, precise, and sensitive RP-HPLC method for the simultaneous determination of acemetacin and chlorzoxazone has been developed. Response surface methodology with a central composite design was used to optimize the acetonitrile and ammonium acetate percentage in the mobile phase and pH of ammonium acetate. The optimum separation was achieved on a C18 column (250 x 4.6 mm id, 5 microm particle size) using the mobile phase methanol-acetonitrile-0.02 M ammonium acetate, pH 9.4 (25 + 35 + 40, v/v/v) at a flow rate of 1.5 mL/min; UV detection at 270 nm, and cyanocobalamin as an internal standard. This developed method was validated and successfully applied to a coated tablet pharmaceutical preparation.

Related Compounds

  • acemetacin
  • Indometacin

Related Articles:

A comparison of low-molecular-weight heparin and combined therapy of low-molecular-weight heparin with an anti-inflammatory agent in the treatment of superficial vein thrombosis.

2009-04-01

[Phlebology 24(2) , 56-60, (2009)]

A fast and reliable spectroscopic method for the determination of membrane--water partition coefficients of organic compounds.

2001-01-01

[Lipids 36(1) , 89-96, (2001)]

Pharmacokinetics of acemetacin and its active metabolite indomethacin in rats during acute hepatic damage and liver regeneration.

2009-01-01

[Ann. Hepatol. 8(2) , 141-7, (2009)]

[Treatment of osteoarthritis of the knee joint. Efficacy and tolerance to acemetacin slow release in comparison to celecoxib].

2004-09-01

[Orthopade. 33(9) , 1032-41, (2004)]

Bartter's Syndrome with Type 2 Diabetes Mellitus

2009-01-01

[J. Chin. Med. Assoc. 72(2) , 88-90, (2009)]

More Articles...