Journal of AOAC International 2013-01-01

HPTLC method for simultaneous analysis of stevioside and rebaudioside-A in Stevia rebaudiana.

Smita V Londhe, Sadhana M Nanaware

Index: J. AOAC Int. 96(1) , 24-6, (2013)

Full Text: HTML

Abstract

An HPTLC method was developed and validated for simultaneous determination of stevioside (STE) and rebaudioside-A (REB-A) in Stevia rebaudiana leaves. The HPTLC separation was performed on precoated silica gel 60F254 HPTLC plates with the mobile phase ethyl acetate-ethanol-acetone-water (15 + 3 + 6 + 6, v/v/v/v). The densitometric quantification of steviol glycosides was carried out at Amax 580 nm in the reflection-absorption mode after spraying with anisaldehyde-sulfuric acid reagent. Rf values were 0.34 and 0.28 for STE and REB-A, respectively. The content of STE and REB-A in leaf extract was found to be 6.94 and 6.35%, respectively. The method was validated in terms of precision, accuracy, specificity, and robustness. The method can be useful for routine analysis of the sweeteners.

Related Compounds

  • Rebaudioside A
  • Stevioside

Related Articles:

Synthesis and sensory evaluation of ent-kaurane diterpene glycosides.

2012-01-01

[Molecules 17(8) , 8908-16, (2012)]

Overview: The history, technical function and safety of rebaudioside A, a naturally occurring steviol glycoside, for use in food and beverages

2008-01-01

[Food Chem. Toxicol. 46 Suppl 7 , S1-S10, (2008)]

Simultaneous determination of steviol and steviol glycosides by liquid chromatography-mass spectrometry.

2012-01-01

[Food Addit. Contam. Part A. Chem. Anal. Control. Expo. Risk Assess. 29(12) , 1861-71, (2012)]

Absolute quantitation of stevioside and rebaudioside A in commercial standards by quantitative NMR.

2013-01-01

[Chem. Pharm. Bull. 61(1) , 33-8, (2013)]

Preparative separation and purification of rebaudioside a from steviol glycosides using mixed-mode macroporous adsorption resins.

2011-09-14

[J. Agric. Food Chem. 59(17) , 9629-36, (2011)]

More Articles...