Bioscience, Biotechnology, and Biochemistry 2004-06-01

Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa).

Kazuyuki Hiwatashi, Kazuyuki Hori, Keitaro Takahashi, Akira Kagaya, Shunzo Inoue, Toshihiro Sugiyama, Saori Takahashi

Index: Biosci. Biotechnol. Biochem. 68 , 1395-7, (2004)

Full Text: HTML

Abstract

We have found a novel prolyl aminopeptidase in Grifola frondosa. The enzyme was purified by DEAE-Sepharose CL-6B, Butyl-Toyopearl, Sephacryl S-100, and Mono-Q column chromatographies. The purified enzyme exists as a dimer and gives high activity toward L-proline-p-nitroanilide. The enzyme was strongly inhibited by p-chloromercuribenzoic acid and iodoacetic acid and markedly inhibited by phenylmethylsulfonyl fluoride and arphamenin A.

Related Compounds

  • h-pro-pna tfa

Related Articles:

Generation of food-grade recombinant Lactobacillus casei delivering Myxococcus xanthus prolyl endopeptidase.

2014-08-01

[Appl. Microbiol. Biotechnol. 98(15) , 6689-700, (2014)]

Synthesis and biological evaluation of nigranoic acid esters as novel human neutrophil elastase inhibitors.

2015-01-01

[Nat. Prod. Res. 29 , 1650-6, (2015)]

Characterization of a multimeric, eukaryotic prolyl aminopeptidase: an inducible and highly specific intracellular peptidase from the non-pathogenic fungus Talaromyces emersonii.

2009-11-01

[Microbiology 155 , 3673-3682, (2009)]

Characterization of an aminopeptidase and a proline iminopeptidase from cabbage leaves.

2008-01-01

[Z. Naturforsch., C, J. Biosci. 63 , 105-112, (2008)]

Collagen production in cardiac fibroblasts during inhibition of aminopeptidase B.

2005-09-01

[J. Renin Angiotensin Aldosterone Syst. 6 , 69-77, (2005)]

More Articles...