[Determination of nequinate and buquinolate in livestock products using liquid chromatography-tandem mass spectrometry].

Takayuki Nakajima, Hiroshi Hayashi, Takeo Sasamoto, Maki Kanda, Tomoko Kusano, Youko Matsushima, Setsuko Kanai, Kazue Takeba, Toshihiro Nagayama

Index: Shokuhin Eiseigaku Zasshi 52(3) , 178-82, (2011)

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Abstract

We studied the simultaneous determination of nequinate and buquinolate, which are used as feed additives to prevent coccidiosis, by means of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The sample was extracted with acetonitrile, then loaded onto an HLB mini-column with 20% methanol. After clean-up with 20% methanol, the analytes were eluted with acetonitrile-methanol (1 : 1). The coccidiostats in the purified samples were determined using ESI-MRM mode LC-MS/MS with a sample matrix calibration curve. Mean recoveries of nequinate and buquinolate from 8 kinds of livestocks samples (chicken muscle, chicken liver, chicken heart, swine muscle, swine heart, cattle muscle, sheep muscle, egg) were in the range of 89.5% to 108.6%, and the relative standard deviation values were <20% (n=10) at the levels of 0.01 μg/g and 0.05 μg/g, respectively. The limits of quantification of these compounds were 0.001 μg/g in each sample.